解剖学报

›› 2009, Vol. 40 ›› Issue (4): 637-641.doi: 10.3969/j.issn.0529-1356.2009.04.023

• 论著 • 上一篇    下一篇

槲皮素和乙酸龙脑酯对细菌脂多糖诱导流产小鼠子宫组织IFN-γ/IL-4和巨噬细胞的影响

王晓丹1，2; 马爱团2 ;史万玉1;姜国均2 ;钟秀会1*   

  1. 1.河北农业大学动物科技学院，河北 保定 071001; 2.河北农业大学中兽医学院，河北 定州 073000
  • 收稿日期:2008-12-27 修回日期:2009-03-05 出版日期:2009-08-06
  • 通讯作者: 钟秀会

Influence of quercetin and bornyl acetate on ratio of IFN-γ/IL-4 and macrophages in uteri of LPS-induced abortion mice

  1. 1.College of Animal Science and Technology, Agricultural University of Hebei, Hebei Baoding 071001, China;2.College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Hebei Dingzhou 073000, China
  • Received:2008-12-27 Revised:2009-03-05 Online:2009-08-06
  • Contact: ZHONG Xiu-hui

关键词: 胚胎丢失, 细菌脂多糖, 巨噬细胞, 干扰素γ, 白细胞介素4, 免疫组织化学, 小鼠

Abstract: Objective To investigate significance of interferon-γ/interleukin-4(IFN-γ/IL-4) and macrophages in uterus in early embryo loss (or resorption), and to elucidate the anti-abortive effect and the immunological modulation at maternal-fetal interface with quercetin and bornvl acetate. Methods Lipopolysaccharide (LPS)（0.10μg/mouse）was injected via tail vein in order to induce abortion in 7-day-gestation mice which received quercetin and bornvl acetate at days 4-7 of gestation. Levels of IFN-γ and IL-4 in uterus lysate supernatant were measured using enzyme-linked immuno-absorbent assay (ELISA), and uterine macrophages of each group (EM>n/EM>=10) were detected by immunohistochemistry. Results The amount of macrophages was much higher in the uteri of LPSinduced abortion mice than that in control mice. The abortion rate of mouse declined to certain level. The therapy of quercetin combined with bornyl acetate reversed LPS-induced abortion. Conclusion The increase of IFN-γ/IL-4 and the amount of macrophages in the LPStreated mouse uterus is associated with the embryo loss, and que

Key words: Embryo resorption, Lipopolysaccharide, Macrophage, IFN-γ, IL-4, Immunohistochemistry, Mouse

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